【英文标准名称】:Abrasivesheets;dimensions,tolerancesanddesignation
【原文标准名称】:砂纸砂布.标识、尺寸和公差
【标准号】:ISO2235-1972
【标准状态】:作废
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【发布单位】:国际标准化组织(IX-ISO)
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【标准类型】:()
【标准水平】:()
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【英文标准名称】:StandardTestMethodforEnumerationofMycobacteriainMetalworkingFluidsbyDirectMicroscopicCounting(DMC)Method
【原文标准名称】:用直接显微镜计数(DMC)法进行金属加工液中分支杆菌计数的标准试验方法
【标准号】:ASTME2564-2007
【标准状态】:现行
【国别】:
【发布日期】:2007
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E34.50
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:计数;流体;工业用油;润滑剂;金属加工;微生物学;测试
【英文主题词】:acid-fastBacteria(AFB);acid-faststaining;directmicroscopiccount(DMC);hypersensitivitypneumonitis(HP);metalworkingfluid(MWF);microscopicfactor(MF);non-tuberculousmycobacterium(NTM)
【摘要】:Duringthepastdecade,ithasbecomeincreasinglyapparentthatnon-tuberculousmycobacteriaarecommonmembersoftheindigenousMWFbacterialpopulation.Measurementofmycobacterialcellcountdensitiesisanimportantstepinestablishingapossiblerelationshipbetweenmycobacteriaandoccupationalhealthrelatedallergicresponses,forexample,HypersensitivityPneumonitis(HP)inpersonsexposedtoaerosolsofmetalworkingfluids.Itisknownthattheviablemycobacteriacountunderestimatesthetotalmycobacteriallevelsbynotcountingthenon-culturable,possiblydeadormoribundpopulationthatispotentiallyequallyimportantintheinvestigationofoccupationalhealthrelatedproblems.TheDirectMicroscopicCountingMethod(DMC)describedheregivesaquantitativeassessmentofthetotalnumbersofacid-fastbacilli.Itinvolvesusingacid-faststainingtoselectivelyidentifymycobacteriafromotherbacteria,followedbyenumerationordirectmicroscopiccountingofaknownvolumeoveraknownarea.Althoughothermicrobesx2014;particularlytheActinomycetesx2014;alsostainacidfast,theyaredifferentiatedfromthemycobacteriabecauseoftheirmorphologyandsize.Non-mycobacteria,acid-fastmicrobesare50-100timeslargerthanmycobacteria.Themethodprovidesquantitativeinformationonthetotal(culturableandnon-culturableviable,andnon-viable)mycobacteriapopulations.TheresultsareexpressedquantitativelyasmycobacteriapermLofmetalworkingfluidsample.TheDMCmethodusingtheacid-faststainingtechniqueisasemi-quantitativemethodwitharelativelyfastturnaroundtime.TheDMCmethodcanalsobeemployedinfieldsurveystudiestocharacterizethechangesintotalmycobacteriadensitiesofmetalworkingfluidsystemsoveralongperiodoftime.ThesensitivitydetectionlimitoftheDMCmethoddependsontheMFandthesamplevolume(directorcentrifuged,etc.)examined.1.1Thistestmethoddescribesadirectmicroscopiccountingmethod(DMC)fortheenumerationoftheacidfaststainedmycobacteriapopulationinmetalworkingfluids.Itcanbeusedtodetectlevelsoftotalmycobacteriapopulation,includingculturableaswellasnon-culturable(possiblydeadormoribund)bacterialcells.Thistestmethodisrecommendedforallwater-basedmetalworkingfluids.1.2Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.Foradditionalsafetyinformation,seeLaboratorySafety:PrincipleandPractices,4thEdition
【中国标准分类号】:H10
【国际标准分类号】:07_100_99;75_100
【页数】:4P.;A4
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